2022年4月，賓夕法尼亞大學(xué)的研究人員在《Journal of Neuroscience》發(fā)表了《Cocaine-Induced Changes in Sperm Cdkn1a Methylation Are Associated with Cocaine Resistance in Male Offspring》棚蓄，文獻(xiàn)的實驗使用了手持式雙波長熒光蛋白觀察手電筒觀察綠色熒光蛋白GFP在小鼠身上的表達(dá)堕扶。DFP-1手持式雙波長熒光蛋白觀察手電筒配合熒光觀察眼鏡能夠快速地觀察熒光蛋白在動物組織器官上的表達(dá)。
DFP-1手持式雙波長熒光蛋白觀察手電筒已經(jīng)停產(chǎn)梭依，如果您對手持式雙波長熒光蛋白觀察手電筒稍算、GFP激發(fā)光源感興趣，請參考美國路陽生產(chǎn)的LUYOR-3415RG雙波長熒光蛋白觀測燈役拴，點擊瀏覽：便攜式熒光蛋白激發(fā)光源LUYOR-3415RG
文獻(xiàn)摘要：
Virally infused rats were given 2.5-3.5 weeks after surgery to ensure knockdown or overexpression of Cdkn1a and expression of GFP control before initiation of cocaine (1.0 mg/kg/inf) self-administration as described above. Rats responded for cocaine on an FR1 schedule for 10 d. The following day, rats were switched to an FR5 schedule for 2 d and then to a PR schedule for 2 d. Under the PR schedule, the response requirement for subsequent drug delivery increased until the session was terminated 30 min after the last completed schedule. The response requirement for the “ith” reinforcement was R(1) = [5e°.2i-5] (Richardson and Roberts, 1996). The breakpoint was operationally defined as the total number of responses for the last completed schedule. Upon completion of behavioral experiments, catheter patency was confirmed by intravenous infusion of ketamine (0.1 ml). Twenty-four hours after the last self-administration session, rats were killed, viral placement was visualized using NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight with NIGHTSEA Barrier Filter Glasses (Electron Microscopy Services), and the fluorescent region of the NAc was harvested.
文獻(xiàn)全文地址：https://doi.org/10.1523/JNEUROSCI.3172-20.2022
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